dot blot hybridization meaning in Chinese
斑点杂交
Examples
- Bl21 . the dot blot hybridization verified that the scfv was secreted to the medium
从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。 - Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。 - The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。 - Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 %
本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析，结果表明： iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性，两者之间仅相差4个核苷酸，同源性高达99 . 7 ，从而证实了iltvtk基因是高度保守的，为iltvtk基因核酸探针的制备提供了有力的依据。